NAD+ 500mg

Description

Nicotinamide Adenine Dinucleotide (NAD+) 500mg Vial – Pharmaceutical Grade Cellular Regeneration Support 

Nicotinamide adenine dinucleotide (NAD⁺) is an essential pyridine nucleotide coenzyme present in virtually all living cells. It plays a central role in cellular metabolism by functioning as an electron carrier in oxidation-reduction (redox) reactions and serves as a required cofactor for numerous enzymes involved in energy production, DNA maintenance, and cellular signaling. Because of its fundamental biological importance, NAD⁺ has become one of the most extensively studied molecules in metabolism, aging biology, and mitochondrial research.

At the molecular level, NAD⁺ participates in glycolysis, the tricarboxylic acid (TCA) cycle, oxidative phosphorylation, and fatty acid metabolism, facilitating the transfer of electrons necessary for ATP production. Beyond its role in cellular bioenergetics, NAD⁺ acts as a substrate for several enzyme families, including sirtuins (SIRT1–7), poly(ADP-ribose) polymerases (PARPs), and cyclic ADP-ribose synthases such as CD38. These enzymes regulate processes including DNA damage responses, chromatin remodeling, inflammatory signaling, calcium homeostasis, circadian biology, and cellular stress adaptation.

Interest in NAD⁺ has increased substantially because intracellular NAD⁺ concentrations decline with aging and in various models of metabolic dysfunction. Experimental research has therefore focused on understanding how modulation of NAD⁺ availability influences mitochondrial function, genomic stability, oxidative stress responses, and age-associated cellular processes. NAD⁺ is widely utilized as a research reagent in molecular biology, biochemistry, pharmacology, and translational biomedical research.

Product Contents

• Nicotinamide Adenine Dinucleotide (NAD+) – 500mg Vial
• Lyophilised (freeze-dried) powder
• 10 Sterile syringes

Product Specifications

• Form: Lyophilised powder
• Total Quantity: 500mg per vial
• Purity: ≥ 98%
• Appearance: White/off-white powder

What Is It Used For and Its Purpose 

NAD⁺ is primarily employed in biomedical research to investigate cellular energy metabolism, mitochondrial physiology, and enzyme systems that depend on intracellular NAD⁺ availability. Because it serves as a universal metabolic cofactor, it is widely used in studies examining how cells generate energy, respond to stress, and maintain genomic integrity.

Researchers investigate NAD⁺ in experimental models of aging biology, neurobiology, metabolic regulation, and DNA repair. Particular emphasis has been placed on understanding how intracellular NAD⁺ concentrations influence mitochondrial function, oxidative stress responses, inflammatory signaling, and cellular resilience during physiological and pathological stress.

NAD⁺ also serves as an important experimental tool for evaluating the activity of NAD⁺-dependent enzymes, including sirtuins and PARPs, both of which play established roles in epigenetic regulation, DNA repair, and cellular adaptation to metabolic demands.

Research Applications

• Endocrinology
• Metabolism
• Mitochondrial function
• Cellular bioenergetics
• Aging research
• Neurobiology
• DNA repair research
• Circadian biology
• Inflammation research
• Biochemistry
• Cellular signaling

Potential Scientific Benefits 

A substantial body of in vitro and preclinical research demonstrates that NAD⁺ is indispensable for cellular energy metabolism. As an electron carrier, it facilitates redox reactions throughout glycolysis, the citric acid cycle, and oxidative phosphorylation, supporting ATP synthesis and mitochondrial respiration. These mechanisms are well established in fundamental biochemistry.

Experimental evidence further indicates that intracellular NAD⁺ availability regulates the activity of sirtuin enzymes and PARPs. Sirtuins influence mitochondrial biogenesis, oxidative stress responses, and metabolic adaptation, whereas PARPs participate in DNA damage detection and repair. Consequently, NAD⁺ depletion may impair multiple pathways involved in maintaining cellular homeostasis.

Studies have investigated whether restoring intracellular NAD⁺ concentrations can influence mitochondrial efficiency, metabolic flexibility, neuroprotection, and age-associated physiological changes. Many experimental findings have shown promising biological effects, outcomes vary depending on species, disease model, dosing strategy, and method of NAD⁺ administration.

• Observed biological activities in research:
  • Support of cellular redox reactions
  • Maintenance of mitochondrial energy metabolism
  • Activation of NAD⁺-dependent enzyme systems
  • Regulation of cellular stress responses
  • Participation in DNA repair mechanisms
• Molecular mechanisms reported:
  • Cofactor for sirtuin enzymes (SIRT family)
  • Substrate for PARP-mediated DNA repair
  • Participation in oxidative phosphorylation
  • Regulation of cellular redox balance
  • Modulation of NAD⁺-dependent signaling pathways
• Physiological pathways involved:
  • Cellular energy metabolism
  • Mitochondrial respiration
  • DNA damage response
  • Oxidative stress regulation
  • Circadian rhythm regulation
  • Cellular aging pathways
• Experimental outcomes in models:
  • Altered mitochondrial function
  • Improved cellular bioenergetics under experimental conditions
  • Modulation of inflammatory signaling
  • Changes in metabolic enzyme activity
  • Enhanced DNA repair capacity in laboratory models

Current evidence remains limited, and further well-controlled clinical studies are required to establish long-term efficacy and safety in humans.

Research Data Sheet 

• Peptide name: Nicotinamide Adenine Dinucleotide (NAD⁺)
• Sequence: Not applicable
• Classification: Endogenous coenzyme; pyridine nucleotide
• Molecular Formula: Not available
• Molecular Weight: Not available
• CAS Number: 53-84-9
• PubChem CID: 5893
• ChemSpider ID: 5681
• ChEMBL ID: CHEMBL4297358
• Form: Sterile solution
• Appearance: Clear aqueous solution
• Solubility: Supplied in aqueous solution
• Storage: Refrigerate after opening; protect from light
• Research Categories: Mitochondrial function, metabolism, aging research, neurobiology, cellular signaling, DNA repair, biochemistry

Safety Precautions 

This NAD⁺ preparation is supplied exclusively for laboratory and scientific research. It must be handled only by trained personnel using appropriate laboratory techniques and institutional safety procedures. It is not intended for diagnostic, therapeutic, veterinary, or human use. Although NAD⁺ is an endogenous biological coenzyme, the safety profile of exogenous research formulations has not been fully characterized for clinical applications. Proper storage, sterile handling, and laboratory documentation should be maintained throughout all experimental procedures.

• Research use only
• Not for human consumption
• Not intended to diagnose, treat, or prevent disease
• Wear appropriate PPE (gloves, laboratory coat, and eye protection)
• Maintain aseptic handling procedures
• Store under recommended refrigerated conditions
• Avoid repeated freeze–thaw cycles where applicable
• Maintain proper labeling and experimental documentation
• Dispose of waste according to institutional and regulatory requirements
• Limited human safety data
• Potential unknown long-term effects

Conclusion 

NAD⁺ is a fundamental biological coenzyme that occupies a central position in cellular metabolism, mitochondrial function, and intracellular signaling. Its involvement in hundreds of enzymatic reactions has established it as one of the most important molecules in modern biomedical research.

Research into NAD⁺ continues to expand across multiple disciplines, including aging biology, metabolism, neurobiology, molecular genetics, and cellular physiology. Its established biochemical roles as a redox cofactor and substrate for NAD⁺-dependent enzymes make it an indispensable research tool for understanding mechanisms underlying cellular resilience, DNA maintenance, and metabolic regulation.

As interest in cellular bioenergetics and healthy aging continues to grow, NAD⁺ remains an essential compound for advancing research into mitochondrial biology, stress adaptation, and the molecular mechanisms that support cellular homeostasis.